New York-Bonhams will offer the 70 guitars and 70 amps next Wednesday.
Among the highlights is a custom-made black Fender 'Eric Clapton' signature Stratocaster, estimated at $20,000 to $30,000. It was used during the "Cream Reunion Shows" in New York and London in 2005.
A pair of 1970 Marshall vintage basket weave speaker cabinets are expected to fetch $8,000-$10,000.
The 65-year-old rock Hall of Famer is a recovered addict who established the non-profit Crossroads Centre in West Indies in 1998.
The British artist's famous love song "Layla" was released in 1970 when he appeared with blues-rock band Derek and The Dominos.