"I'd love to thank my family for all their love and support including my husband," she said at the time, as the hip-hop mogul sat, surprised, in the audience.
"He was shocked!" Beyonce told Access. "He was just like, 'What did you say?'"
And the singer added that she couldn't believe it herself.
"I was just like, 'What did I just do? No, I didn't just say that,'" she said, explaining that the outburst came in the heat of the moment.
"I was so happy, I could've said anything," she said of taking the stage for her win for Female Pop Vocal – her sixth Grammy of the night, a record for a female artist. "That's why I ran off the stage."
She also took home a top honor with Song of the Year for "Single Ladies (Put A Ring On It)," a songwriting category.
So what did Jay-Z say back?
"He said you have six Grammys and you smell good," she laughed.
The singer was wearing her new scent, Beyonce Heat, for which she recently filmed a steamy ad – and the perfume is already a big hit.
"This morning I woke up and they told me the fragrance is the number one fragrance in America," Beyonce told Access. "I had absolutely no idea."
And filming the spot may have taken some double-stick tape to keep her dress in place, but the singer added that it wasn't all hard work.
"It was my birthday when we filmed the video," she said. "We had fun. They brought out cake and candles."
Related Content from AccessHollywood.com:
VIEW THE PHOTOS: We Like What We See Of Beyonce!
PLAY IT NOW: Behind The Scenes: Beyonce Tackles The Super Bowl
PLAY IT NOW: 2010 Grammy Awards: Backstage With Jay-Z